Journal: Communications Biology

Article Title: Consolidation of cell-ECM adhesion through direct talin-mediated actin linkage is essential for mouse embryonic morphogenesis

doi: 10.1038/s42003-025-08294-3

Figure Lengend Snippet: A Representative images of MEFs of each genotype co-stained with a total β1-integrin antibody (yellow) and an active β1-integrin antibody 9EG7 (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. B Intensity ratios of active β1-integrin 9EG7 over total β1-integrin in MEFs of each genotype at both time points. n = 50 cells per genotype for each time point. C Representative images of MEFs of each genotype co-stained with a paxillin antibody (yellow) and a total β1-integrin antibody (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. D Pearson correlation coefficient of paxillin and total β1-integrin in MEFs of each genotype at both time points. n = 48–52 cells per genotype for each time point. E Representative images of MEFs of each genotype co-stained with a paxillin antibody (yellow) and an active β1-integrin antibody 9EG7 (magenta). Cells were spread for 30 min (left panel) and 90 min (right panel), respectively. Scale bar = 20 μm. F Pearson correlation coefficient of paxillin and active β1-integrin 9EG7 in MEFs of each genotype at both time points. n = 50–56 cells per genotype for each time point. P values were determined using Ordinary one-way ANOVA, Dunnett’s multiple comparisons test. *** p ≤ 0.0001, ** p ≤ 0.001, and * p ≤ 0.01.

Article Snippet: Cells were incubated with primary antibodies goat anti-total β1-integrin (1:500, R&D Systems, AF2405) and rat anti-active β1-integrin 9EG7 (1:10, BD Biosciences, 550531) diluted in flow buffer for 1 h on ice followed by three washes with flow buffer.

Techniques: Staining